CLIP-seq iCount Merge Summary GTF error

Hi! I’m trying to run my recent sequencing results through the CLIP-seq pipeline but I keep getting the same error - I’ve included screen shots from the Report and the terminal for specifics. I tried running different samples from this batch of sequencing but no difference. In case it was an issue with demultiplexing, I re-ran one of my samples from November that I have previously run successfully, but the error was still present for the CLIP-seq run, so I’m wondering if there’s something going on with the pipeline? Or if there’s something I need to do differently that I haven’t realized yet with the gtf.

Happy to answer any questions to troubleshoot this and appreciate your assistance in advance!

Hi Megan,

We’ve seen similar reports from a couple of users when using Nextflow 25 with this pipeline - could you try running it again but change the Nextflow version to 24.10.8?

Thanks,

Alex

That did seem to do the trick! Thank you!